Extracellular vesicles isolation and standardisation

Isolation and characterization of extracellular vesicles

DOI: https://doi.org/10.47184/tev.2019.01.02

Since EVs are secreted by most, if not all, eukaryotic and prokaryotic cells, they have been detected in body fluids as diverse as blood, urine and saliva as well as in cell culture media. This manuscript gives an overview of EV isolation and characterization strategies and highlight their advantages and disadvantages.

Keywords: extracellular vesicle isolation, purification, characterization, exosome

EV isolation: finding the needle in the haystack

The fundamental goal of a successful isolation method entails both the efficient enrichment of EVs from a given biofluid and the depletion of non-EV material, yielding preparations of high purity without significant loss of EVs (Figure 1). 

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Authors
Fabia Fricke
Department of Applied Tumor Biology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany
Clinical Cooperation Unit Applied Tumor Biology, German Cancer Research Centre (DKFZ), Heidelberg, Germany
Michael W. Pfaffl
Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan
Technical University of Munich, Freising, Germany